View source for PhiX:PhiX Methods

The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

==Protocol pages==
[[PhiX:Stock preparation | Preparing High Titer Stock]]<br>
[[PhiX:Serial dilution | Titering/Serial dilution]]<br>
[[PhiX:Plaque assay | Double Agar Plaque Assay]]<br>
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>
[[PhiX:DNA precipitation | DNA precipitation]]<br>
[[PhiX:Mutagenesis | Mutagenesis]]<br>
[[PhiX:Transformation | Competent cell preparation and transformation]]

==Other useful pages==
[[PhiX:Recipes | Buffer recipes]]<br>
[[PhiX:The non-toxic lab | The non-toxic lab]]<br>
[http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page]<br>
[http://bendickins.net Dickins Lab Pages]

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