PhiX:PhiX Methods

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==Microviridae Protocols: editable methods for φX174 (ssDNA phage)==
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The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.
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Protocol Pages:<br>[[PhiX:Recipes | Buffer recipes]]<br>[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
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[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>[[PhiX:DNA precipitation]]<br>[[PhiX:Mutagenesis]]<br>
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[[PhiX:Transformation]]
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Another page of some interest for lab methods is [[PhiX:The non-toxic lab | this one]] which aims to aggregate safer and more convenient lab methods for simple frequently carried out lab procedures.
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==Protocol pages==
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[[PhiX:Stock preparation | Preparing High Titer Stock]]<br>
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[[PhiX:Serial dilution | Titering/Serial dilution]]<br>
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[[PhiX:Plaque assay | Double Agar Plaque Assay]]<br>
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[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
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[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>
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[[PhiX:DNA precipitation | DNA precipitation]]<br>
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[[PhiX:Mutagenesis | Mutagenesis]]<br>
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[[PhiX:Transformation | Competent cell preparation and transformation]]
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==How to edit this stuff==
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==Other useful pages==
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Consult the [http://meta.wikimedia.org/wiki/Help:Contents User's Guide] for information on using the wiki software. Also:
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[[PhiX:Recipes | Buffer recipes]]<br>
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[[PhiX:The non-toxic lab | The non-toxic lab]]<br>
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[http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page]<br>
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[http://bendickins.net Dickins Lab Pages]
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==How to contribute==
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Consult the [http://meta.wikimedia.org/wiki/Help:Contents User's Guide] for information on using wiki software. Also:
* [http://www.mediawiki.org/wiki/Manual:Configuration_settings Configuration settings list]
* [http://www.mediawiki.org/wiki/Manual:Configuration_settings Configuration settings list]
* [http://www.mediawiki.org/wiki/Manual:FAQ MediaWiki FAQ]
* [http://www.mediawiki.org/wiki/Manual:FAQ MediaWiki FAQ]
* [http://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list]
* [http://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list]

Latest revision as of 23:19, 22 February 2017

The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

Protocol pages

Preparing High Titer Stock
Titering/Serial dilution
Double Agar Plaque Assay
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation

Other useful pages

Buffer recipes
The non-toxic lab
Wikipedia's φX174 page
Dickins Lab Pages

How to contribute

Consult the User's Guide for information on using wiki software. Also:

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