PhiX:PhiX Methods

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==Protocol pages==
==Protocol pages==
 +
[[PhiX:Stock preparation | Preparing High Titer Stock]]<br>
 +
[[PhiX:Serial dilution | Titering/Serial dilution]]<br>
 +
[[PhiX:Plaque assay | Double Agar Plaque Assay]]<br>
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
-
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>[[PhiX:DNA precipitation]]<br>[[PhiX:Mutagenesis]]<br>
+
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>
-
[[PhiX:Transformation]]
+
[[PhiX:DNA precipitation | DNA precipitation]]<br>
 +
[[PhiX:Mutagenesis | Mutagenesis]]<br>
 +
[[PhiX:Transformation | Competent cell preparation and transformation]]
==Other useful pages==
==Other useful pages==
-
[[PhiX:Recipes | Buffer recipes]]<br>[[PhiX:The non-toxic lab]]<br>
+
[[PhiX:Recipes | Buffer recipes]]<br>
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[http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page]
+
[[PhiX:The non-toxic lab | The non-toxic lab]]<br>
 +
[http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page]<br>
 +
[http://bendickins.net Dickins Lab Pages]
==How to contribute==
==How to contribute==

Latest revision as of 23:19, 22 February 2017

The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

Protocol pages

Preparing High Titer Stock
Titering/Serial dilution
Double Agar Plaque Assay
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation

Other useful pages

Buffer recipes
The non-toxic lab
Wikipedia's φX174 page
Dickins Lab Pages

How to contribute

Consult the User's Guide for information on using wiki software. Also:

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