Difference between revisions of "PhiX:PhiX Methods"
From CCGB
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[[PhiX:The non-toxic lab | The non-toxic lab]]<br> | [[PhiX:The non-toxic lab | The non-toxic lab]]<br> | ||
[http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page] | [http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page] | ||
| + | [http://bendickins.net Dickins Lab Pages] | ||
==How to contribute== | ==How to contribute== | ||
Revision as of 12:08, 20 February 2017
The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.
Protocol pages
Titering/Serial dilution
Double Agar Plaque Assay
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation
Other useful pages
Buffer recipes
The non-toxic lab
Wikipedia's φX174 page
Dickins Lab Pages
How to contribute
Consult the User's Guide for information on using wiki software. Also:
