PhiX:Recipes
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Revision as of 15:53, 14 August 2009 by Ben (talk) (Created page with ''''BE Buffer (one litre; 50mM Na<sub>2</sub>B<sub>4</sub>O<sub>7</sub>; 5.0mM EDTA)'''<br> 19.1g Na<sub>2</sub>B<sub>4</sub>O<sub>7</sub>.10H<sub>2</sub>O<br> 1.86g EDTA (disodiu…')
BE Buffer (one litre; 50mM Na2B4O7; 5.0mM EDTA)
19.1g Na2B4O7.10H2O
1.86g EDTA (disodium salt)
water to 1 litre
Tris-saturated Phenol with antioxidant (with 100ml phenol)
add 100mg 8-hydroxyquinoline (i.e., 0.1% w/v)
add equilibration buffer (-> pH 7.9) - 6.5ml of this in Sigma kit
add 130ul 2-mercaptoethanol (0.2% of buffer phase)
EDTA stock (0.5M)
use disodium salt and pH to 8 with NaOH
SDS 20%
pre-bought - avoids fine powder
Tris-HCl stock (1M)
pH to 8 with HCl
Pronase to 20mg/ml
dissolve in nuclease-free water and store at -20oC
