Difference between revisions of "PhiX:Serial dilution"
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(Created page with "==SUMMARY== '''PHAGE SOLUTION''' Titer = x pfu/ml OR y pfu in z ul '''SERIAL DILUTION''' Add 100ul (10<sup>-1</sup>x) phage to 900ul HFB and mix = TUBE #1 Transfer 100ul (10<su...") |
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==SUMMARY== | ==SUMMARY== | ||
| − | '''PHAGE SOLUTION''' | + | '''PHAGE SOLUTION'''<br> |
| − | Titer = x pfu/ml | + | Titer = x pfu/ml<br> |
OR y pfu in z ul | OR y pfu in z ul | ||
| − | '''SERIAL DILUTION''' | + | '''SERIAL DILUTION'''<br> |
| − | Add 100ul (10<sup>-1</sup>x) phage to 900ul HFB and mix = TUBE #1 | + | Add 100ul (10<sup>-1</sup>x) phage to 900ul HFB and mix = TUBE #1<br> |
| − | Transfer 100ul (10<sup>-2</sup>x) #1 to 900ul HFB and mix = TUBE #2 | + | Transfer 100ul (10<sup>-2</sup>x) #1 to 900ul HFB and mix = TUBE #2<br> |
| − | Transfer 100ul (10<sup>-3</sup>x) #2 to 900ul HFB and mix = TUBE #3 | + | Transfer 100ul (10<sup>-3</sup>x) #2 to 900ul HFB and mix = TUBE #3<br> |
and so on... | and so on... | ||
| − | '''PLATING''' | + | '''PLATING'''<br> |
| − | TUBE #1 -> plate 100ul (1/10) -> 10<sup>-2</sup>x | + | TUBE #1 -> plate 100ul (1/10) -> 10<sup>-2</sup>x<br> |
| − | TUBE #2 -> plate 100ul (1/10) -> 10<sup>-3</sup>x | + | TUBE #2 -> plate 100ul (1/10) -> 10<sup>-3</sup>x<br> |
| − | TUBE #3 -> plate 100ul (1/10) -> 10<sup>-4</sup>x | + | TUBE #3 -> plate 100ul (1/10) -> 10<sup>-4</sup>x<br> |
and so on... | and so on... | ||
| − | '''MATHS''' | + | '''MATHS'''<br> |
| − | Count n plaques on plate from 10<sup>-7</sup>x plate: | + | Count n plaques on plate from 10<sup>-7</sup>x plate:<br> |
| − | x = n * 10<sup>-7</sup> | + | x = n * 10<sup>-7</sup><br> |
| − | OR if z = 200ul (e.g., mutagenesis) | + | OR if z = 200ul (e.g., mutagenesis)<br> |
y = (n * 10<sup>-7</sup>) * z/1000 | y = (n * 10<sup>-7</sup>) * z/1000 | ||
Revision as of 20:11, 6 August 2013
SUMMARY
PHAGE SOLUTION
Titer = x pfu/ml
OR y pfu in z ul
SERIAL DILUTION
Add 100ul (10-1x) phage to 900ul HFB and mix = TUBE #1
Transfer 100ul (10-2x) #1 to 900ul HFB and mix = TUBE #2
Transfer 100ul (10-3x) #2 to 900ul HFB and mix = TUBE #3
and so on...
PLATING
TUBE #1 -> plate 100ul (1/10) -> 10-2x
TUBE #2 -> plate 100ul (1/10) -> 10-3x
TUBE #3 -> plate 100ul (1/10) -> 10-4x
and so on...
MATHS
Count n plaques on plate from 10-7x plate:
x = n * 10-7
OR if z = 200ul (e.g., mutagenesis)
y = (n * 10-7) * z/1000
