Difference between revisions of "PhiX:Serial dilution"
From CCGB
m |
(→Summary) |
||
| Line 22: | Line 22: | ||
OR, if z = 200ul (e.g., mutagenesis)<br> | OR, if z = 200ul (e.g., mutagenesis)<br> | ||
y = (n * 10<sup>-7</sup>) * 200/1000 | y = (n * 10<sup>-7</sup>) * 200/1000 | ||
| + | |||
| + | [[File:dilution.jpg]] | ||
Revision as of 06:23, 20 February 2017
Summary
PHAGE SOLUTION
Titer = x pfu/ml
OR = y pfu in z ul
SERIAL DILUTION
Add 100ul (10-1x) phage to 900ul HFB and mix -> tube #1
Transfer 100ul (10-2x) #1 to 900ul HFB and mix -> tube #2
Transfer 100ul (10-3x) #2 to 900ul HFB and mix -> tube #3
and so on...
PLATING
Tube #1 -> plate 100ul (1/10) -> 10-2x
Tube #2 -> plate 100ul (1/10) -> 10-3x
Tube #3 -> plate 100ul (1/10) -> 10-4x
and so on...
MATHS
E.g. count n plaques on plate from 10-7x plate:
x = n * 10-7
OR, if z = 200ul (e.g., mutagenesis)
y = (n * 10-7) * 200/1000
