PhiX:Serial dilution
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Revision as of 20:10, 6 August 2013 by Ben (talk | contribs) (Created page with "==SUMMARY== '''PHAGE SOLUTION''' Titer = x pfu/ml OR y pfu in z ul '''SERIAL DILUTION''' Add 100ul (10<sup>-1</sup>x) phage to 900ul HFB and mix = TUBE #1 Transfer 100ul (10<su...")
SUMMARY
PHAGE SOLUTION Titer = x pfu/ml OR y pfu in z ul
SERIAL DILUTION Add 100ul (10-1x) phage to 900ul HFB and mix = TUBE #1 Transfer 100ul (10-2x) #1 to 900ul HFB and mix = TUBE #2 Transfer 100ul (10-3x) #2 to 900ul HFB and mix = TUBE #3 and so on...
PLATING TUBE #1 -> plate 100ul (1/10) -> 10-2x TUBE #2 -> plate 100ul (1/10) -> 10-3x TUBE #3 -> plate 100ul (1/10) -> 10-4x and so on...
MATHS Count n plaques on plate from 10-7x plate: x = n * 10-7 OR if z = 200ul (e.g., mutagenesis) y = (n * 10-7) * z/1000
