PhiX:Serial dilution

SUMMARY

PHAGE SOLUTION
Titer = x pfu/ml
OR y pfu in z ul

SERIAL DILUTION
Add 100ul (10^-1x) phage to 900ul HFB and mix = TUBE #1
Transfer 100ul (10^-2x) #1 to 900ul HFB and mix = TUBE #2
Transfer 100ul (10^-3x) #2 to 900ul HFB and mix = TUBE #3
and so on...

PLATING
TUBE #1 -> plate 100ul (1/10) -> 10^-2x
TUBE #2 -> plate 100ul (1/10) -> 10^-3x
TUBE #3 -> plate 100ul (1/10) -> 10^-4x
and so on...

MATHS
Count n plaques on plate from 10^-7x plate:
x = n * 10^-7
OR if z = 200ul (e.g., mutagenesis)
y = (n * 10^-7) * z/1000