View source for PhiX:Serial dilution

==Summary==

'''PHAGE SOLUTION'''<br>
Titer = x pfu/ml<br>
OR =  y pfu in z ul<br>

'''SERIAL DILUTION'''<br>
Add 100ul (10<sup>-1</sup>x) phage to 900ul HFB and mix -> tube #1<br>
Transfer 100ul (10<sup>-2</sup>x) #1 to 900ul HFB and mix -> tube #2<br>
Transfer 100ul (10<sup>-3</sup>x) #2 to 900ul HFB and mix -> tube #3<br>
and so on...<br>

'''PLATING'''<br>
Tube #1 -> plate 100ul (1/10) -> 10<sup>-2</sup>x<br>
Tube #2 -> plate 100ul (1/10) -> 10<sup>-3</sup>x<br>
Tube #3 -> plate 100ul (1/10) -> 10<sup>-4</sup>x<br>
and so on...<br>

'''MATHS'''<br>
E.g. count n plaques on plate from 10<sup>-7</sup>x plate:<br>
x = n * 10<sup>-7</sup><br>
OR, if z = 200ul (e.g., mutagenesis)<br>
y = (n * 10<sup>-7</sup>) * 200/1000

[[File:dilution.jpg]]