Difference between revisions of "PhiX:PhiX Methods"
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The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community. | The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community. | ||
| + | ==Protocol pages== | ||
| + | [[PhiX:Stock preparation | Preparing High Titer Stock]]<br> | ||
| + | [[PhiX:Serial dilution | Titering/Serial dilution]]<br> | ||
| + | [[PhiX:Plaque assay | Double Agar Plaque Assay]]<br> | ||
| + | [[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br> | ||
| + | [[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br> | ||
| + | [[PhiX:DNA precipitation | DNA precipitation]]<br> | ||
| + | [[PhiX:Mutagenesis | Mutagenesis]]<br> | ||
| + | [[PhiX:Transformation | Competent cell preparation and transformation]] | ||
| − | == | + | ==Other useful pages== |
| − | [[PhiX:Recipes | Buffer recipes]]<br>[[PhiX: | + | [[PhiX:Recipes | Buffer recipes]]<br> |
| − | [ | + | [[PhiX:The non-toxic lab | The non-toxic lab]]<br> |
| − | [ | + | [http://en.wikipedia.org/wiki/Phi_X_174 Wikipedia's φX174 page]<br> |
| + | [http://bendickins.net Dickins Lab Pages] | ||
| − | + | ==How to contribute== | |
| − | + | Consult the [http://meta.wikimedia.org/wiki/Help:Contents User's Guide] for information on using wiki software. Also: | |
| − | ==How to | ||
| − | Consult the [http://meta.wikimedia.org/wiki/Help:Contents User's Guide] for information on using | ||
* [http://www.mediawiki.org/wiki/Manual:Configuration_settings Configuration settings list] | * [http://www.mediawiki.org/wiki/Manual:Configuration_settings Configuration settings list] | ||
* [http://www.mediawiki.org/wiki/Manual:FAQ MediaWiki FAQ] | * [http://www.mediawiki.org/wiki/Manual:FAQ MediaWiki FAQ] | ||
* [http://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list] | * [http://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list] | ||
Latest revision as of 18:19, 22 February 2017
The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.
Protocol pages
Preparing High Titer Stock
Titering/Serial dilution
Double Agar Plaque Assay
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation
Other useful pages
Buffer recipes
The non-toxic lab
Wikipedia's φX174 page
Dickins Lab Pages
How to contribute
Consult the User's Guide for information on using wiki software. Also:
