Difference between revisions of "PhiX:DNA precipitation"

Latest revision as of 15:54, 22 February 2010

• 1 volume of isopropanol or 2.5 volumes of 100% ethanol
• 0.1 volume 5M NaCl (or NaAc to 0.3M)
• one hour at -80^oC or O/N at -20^oC
• warm to 0^oC (on ice; else viscosity impairs spin)
• centrifuge at full speed for 15 minutes
• wash pellet in 70% ethanol (this removes salt more efficiently than isopropanol)
• re-suspend in 10mM Tris, 1mM EDTA, pH 7.5 or in water - may need one hour at 37^oC

Remember that isopropanol is a little more efficient (needs less coolth/time to precipitate) but that it precipitates more salt.