Difference between revisions of "PhiX:PhiX Methods"

From CCGB
Jump to: navigation, search
Line 2: Line 2:
  
 
==Protocol pages==
 
==Protocol pages==
[[PhiX:Serial dilution | Serial dilution]]<br>
+
[[PhiX:Serial dilution | Titering/Serial dilution]]<br>
 
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
 
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
 
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>
 
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>

Revision as of 19:28, 6 August 2013

The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

Protocol pages

Titering/Serial dilution
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation

Other useful pages

Buffer recipes
The non-toxic lab
Wikipedia's φX174 page

How to contribute

Consult the User's Guide for information on using wiki software. Also: