Difference between revisions of "PhiX:Serial dilution"
From CCGB
| Line 1: | Line 1: | ||
| − | == | + | ==Summary== |
'''PHAGE SOLUTION'''<br> | '''PHAGE SOLUTION'''<br> | ||
Titer = x pfu/ml<br> | Titer = x pfu/ml<br> | ||
| − | OR y pfu in z ul | + | OR = y pfu in z ul |
'''SERIAL DILUTION'''<br> | '''SERIAL DILUTION'''<br> | ||
| Line 18: | Line 18: | ||
'''MATHS'''<br> | '''MATHS'''<br> | ||
| − | + | E.g. count n plaques on plate from 10<sup>-7</sup>x plate:<br> | |
x = n * 10<sup>-7</sup><br> | x = n * 10<sup>-7</sup><br> | ||
| − | OR if z = 200ul (e.g., mutagenesis)<br> | + | OR, if z = 200ul (e.g., mutagenesis)<br> |
y = (n * 10<sup>-7</sup>) * z/1000 | y = (n * 10<sup>-7</sup>) * z/1000 | ||
Revision as of 19:12, 6 August 2013
Summary
PHAGE SOLUTION
Titer = x pfu/ml
OR = y pfu in z ul
SERIAL DILUTION
Add 100ul (10-1x) phage to 900ul HFB and mix = TUBE #1
Transfer 100ul (10-2x) #1 to 900ul HFB and mix = TUBE #2
Transfer 100ul (10-3x) #2 to 900ul HFB and mix = TUBE #3
and so on...
PLATING
TUBE #1 -> plate 100ul (1/10) -> 10-2x
TUBE #2 -> plate 100ul (1/10) -> 10-3x
TUBE #3 -> plate 100ul (1/10) -> 10-4x
and so on...
MATHS
E.g. count n plaques on plate from 10-7x plate:
x = n * 10-7
OR, if z = 200ul (e.g., mutagenesis)
y = (n * 10-7) * z/1000
