Difference between revisions of "PhiX:Serial dilution"
From CCGB
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x = n * 10<sup>-7</sup><br> | x = n * 10<sup>-7</sup><br> | ||
OR, if z = 200ul (e.g., mutagenesis)<br> | OR, if z = 200ul (e.g., mutagenesis)<br> | ||
| − | y = (n * 10<sup>-7</sup>) * | + | y = (n * 10<sup>-7</sup>) * 200/1000 |
Revision as of 19:14, 6 August 2013
Summary
PHAGE SOLUTION
Titer = x pfu/ml
OR = y pfu in z ul
SERIAL DILUTION
Add 100ul (10-1x) phage to 900ul HFB and mix -> tube #1
Transfer 100ul (10-2x) #1 to 900ul HFB and mix -> tube #2
Transfer 100ul (10-3x) #2 to 900ul HFB and mix -> tube #3
and so on...
PLATING
Tube #1 -> plate 100ul (1/10) -> 10-2x
Tube #2 -> plate 100ul (1/10) -> 10-3x
Tube #3 -> plate 100ul (1/10) -> 10-4x
and so on...
MATHS
E.g. count n plaques on plate from 10-7x plate:
x = n * 10-7
OR, if z = 200ul (e.g., mutagenesis)
y = (n * 10-7) * 200/1000
