Difference between revisions of "PhiX:PhiX Methods"
From CCGB
| Line 4: | Line 4: | ||
[[PhiX:Serial dilution | Serial dilution]]<br> | [[PhiX:Serial dilution | Serial dilution]]<br> | ||
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br> | [[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br> | ||
| − | [[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>[[PhiX:DNA precipitation]]<br>[[PhiX:Mutagenesis]]<br> | + | [[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br> |
| + | [[PhiX:DNA precipitation | DNA precipitation]]<br> | ||
| + | [[PhiX:Mutagenesis | Mutagenesis]]<br> | ||
[[PhiX:Transformation | Competent cell preparation and transformation]] | [[PhiX:Transformation | Competent cell preparation and transformation]] | ||
Revision as of 19:18, 6 August 2013
The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.
Protocol pages
Serial dilution
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation
Other useful pages
Buffer recipes
PhiX:The non-toxic lab
Wikipedia's φX174 page
How to contribute
Consult the User's Guide for information on using wiki software. Also:
