PhiX:PhiX Methods
From CCGB
The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.
Protocol pages
Serial dilution
Single-stranded DNA isolation
Double-stranded DNA isolation
PhiX:DNA precipitation
PhiX:Mutagenesis
Competent cell preparation and transformation
Other useful pages
Buffer recipes
PhiX:The non-toxic lab
Wikipedia's φX174 page
How to contribute
Consult the User's Guide for information on using wiki software. Also:
