SLab:Run Processing

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Revision as of 16:47, 25 March 2010 by Rico (talk | contribs) (signal processing)

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454

rigs
  • schuster-flx1
  • schuster-flx2
  • schuster-flx3
  • schuster-flx4

on-rig processing

  • run directories are stored in /data
    • /data/YYYY_MM_DD/R_YYYY_MM_DD_HH_MM_SS_RIGNAME_OPERATOR_RUNNAME
  • when a run finishes processing, it calls the /usr/local/rig/bin/postAnalysisScript.sh script
    • rsync's run directory to s2:/zfs/md1k-4/data/sequencing/temp/454
    • ssh's to c1.persephone to submit job
      • depending on run
        • calls c1.persephone:/usr/local/bin/submit-signalProcessing.sh
        • calls c1.persephone:/usr/local/bin/submit-fullProcessing.sh
    • status email is sent to 454pipeline@bx.psu.edu
    • our postAnalysisScript.sh is kept in /home/adminrig/postAnalysisScript directory on each rig
      • revision controlled using rcs
        •  % co -l postAnalysisScript.sh
        •  % vi postAnalysisScript.sh
        •  % ci -u postAnalysisScript.sh
      • Makefile in this directory installs our version into /usr/local/rig/bin
        •  % make install

signal processing

  • signal processing for runs is performed on the persephone cluster
  • depending on run
    • uses qsub to submit job using c1.persephone:/usr/local/bin/signalProcessing.qsub
    • uses qsub to submit job using c1.persephone:/usr/local/bin/fullProcessing.qsub
  • status email is sent to 454pipeline@bx.psu.edu

Before exiting signal processing jobs signal that processing is done by touching a file with the same name as the run directory:

/afs/bx.psu.edu/depot/data/schuster_lab/sequencing/temp/454/.processing_finished/RUN_DIR_NAME

Illumina

systems
  • illumina-ga