PhiX:Recipes

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SB Buffer for high-speed, low-temp agarose electrophoresis
45g boric acid
8g sodium hydroxide pellets
water to 1 litre

BE Buffer (one litre; 50mM Na2B4O7; 5.0mM EDTA)
19.1g Na2B4O7.10H2O
1.86g EDTA (disodium salt)
water to 1 litre

HFB Buffer (60mM NH4Cl; 90mM NaCl; 0.1M KCl; 0.1M Tris-HCl (pH 7.4); 1.0mM MgSO4; 1.0mM CaCl2)
For 500ml 10× stock:
125ml of 2.0 M Tris-HCl (pH 7.3)
15g NH4Cl
2.5g NaCl
25g KCl
5.0ml 1.0 M MgSO4
5.0ml 1.0M CaCl2
water up to 500ml
For working solution:
dilute to 1× in water and autoclave

Tris-saturated Phenol with antioxidant (with 100ml phenol)
add 100mg 8-hydroxyquinoline (i.e., 0.1% w/v)
add equilibration buffer (-> pH 7.9) - 6.5ml of this in Sigma kit
add 130ul 2-mercaptoethanol (0.2% of buffer phase)

EDTA stock (0.5M)
use disodium salt and pH to 8 with NaOH

SDS 20%
pre-bought - avoids fine powder

Tris-HCl stock (1M)
pH to 8 with HCl

Pronase to 20mg/ml
dissolve in nuclease-free water and store at -20oC

10 X PE1
200mM Tris pH 7.5
100mM MgCl2
200 mM NaCl
10 mM DTT

10 X PE2
200 mM Tris pH 7.5
100 mM MgCl2
100 mM DTT