PhiX:Serial dilution

Summary

PHAGE SOLUTION
Titer = x pfu/ml
OR = y pfu in z ul

SERIAL DILUTION
Add 100ul (10^-1x) phage to 900ul HFB and mix -> tube #1
Transfer 100ul (10^-2x) #1 to 900ul HFB and mix -> tube #2
Transfer 100ul (10^-3x) #2 to 900ul HFB and mix -> tube #3
and so on...

PLATING
Tube #1 -> plate 100ul (1/10) -> 10^-2x
Tube #2 -> plate 100ul (1/10) -> 10^-3x
Tube #3 -> plate 100ul (1/10) -> 10^-4x
and so on...

MATHS
E.g. count n plaques on plate from 10^-7x plate:
x = n * 10^-7
OR, if z = 200ul (e.g., mutagenesis)
y = (n * 10^-7) * 200/1000

File:Dilution.pdf