PhiX:SsDNA isolation

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Full credit to Bentley Fane for base protocol.

Day One: phage amplification

(prepare 1M MgCl2)

(phage will attach to cell debris o/n; fix shaker platform; check stuff for tomorrow)

Day Two: phage detachment

(prepare BE buffer)

(phage will detach from cell debris in presence of EDTA in BE)

Day Three: ssDNA preparation

(check BE pH - Roche pronase says pH7.5 is nice + Tris)

Nanodrop spec and gel run for purity and concentration

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