PhiX:PhiX Methods

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The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

Protocol pages

Preparing High Titer Stock
Titering/Serial dilution
Double Agar Plaque Assay
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation

Other useful pages

Buffer recipes
The non-toxic lab
Wikipedia's φX174 page
Dickins Lab Pages

How to contribute

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