PhiX:PhiX Methods

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[[PhiX:Serial dilution | Serial dilution]]<br>
[[PhiX:Serial dilution | Serial dilution]]<br>
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
[[PhiX:ssDNA isolation | Single-stranded DNA isolation]]<br>
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[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>[[PhiX:DNA precipitation]]<br>[[PhiX:Mutagenesis]]<br>
+
[[PhiX:dsDNA isolation | Double-stranded DNA isolation]]<br>
 +
[[PhiX:DNA precipitation | DNA precipitation]]<br>
 +
[[PhiX:Mutagenesis | Mutagenesis]]<br>
[[PhiX:Transformation | Competent cell preparation and transformation]]
[[PhiX:Transformation | Competent cell preparation and transformation]]

Revision as of 00:18, 7 August 2013

The aim of this wiki is to collate and finesse protocols, old, new, borrowed, and blue, that can be used to manipulate φX174 and related Microviridae. Handling these tricky critters rewards some attention to detail and I hope this website becomes a valuable source of those details for the φX174 community.

Protocol pages

Serial dilution
Single-stranded DNA isolation
Double-stranded DNA isolation
DNA precipitation
Mutagenesis
Competent cell preparation and transformation

Other useful pages

Buffer recipes
PhiX:The non-toxic lab
Wikipedia's φX174 page

How to contribute

Consult the User's Guide for information on using wiki software. Also:

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